Kurs: Introduction to Stem Cell Biology– (WBT-400E)


  • dr hab. Ewa Zuba-Surma, prof. UJ (lectures)
  • dr hab. Ewa Zuba-Surma, prof. UJ (practical classes coordinator and leader)
  • mgr Anna Łabędź-Masłowska (practical classes assistant)

Hours: 15 h lectures + 15 h practical classes


Semester: summer

Place »

  • Lectures:                 Rm D105
  • Practical classes:      Rm C110

Dates »

  • Lectures:
    • Thursday          10.00–11.30am
  • Practical classes:
    • Wednesday      9.00–12.45

Initial Requirements:

This course is open for students who know English in reading, writing and speaking.
Completed "Cell Biology" course WBt-BT165 will be preffered (not applicable for international and ERASMUS students).


  • REPORT from practical classes
  • Final EXAM with grade - including information from lectures and practical classes

Basic info:

  • The course is dedicated for Polish as well as international students and ERASMUS Programme participants.
  • All lectures, discussions and final test are given in English

Description »

Course given in ENGLISH.

The aim of the course would be to introduce basic aspects of stem cell (SC) biology and regenerative medicine to the participants .

The course will introduce: i) major populations of SC, ii) distinct sources for SC isolation including those used for clinical purposes, iii) genetic methods of SC modifications, iv) methods for SC identification and purification; v) practical applications of SC and their derivatives in regenerative medicine and for diagnostics.

  • Lectures include:
  • Stem/ progenitor cell types (e.g embryonic, fetal and adult SC, iPS cells);
  • Sources for SC isolations including embryonic and adult tissues; (e.g. bone marrow, cord blood, mobilized peripheral blood);
  • Methods for genetic reprogramming of somatic cells into iPS cells and direct progenitors;
  • Approaches for identification and characterisation of rare SC populations (including the role of classical and imaging cytometry);
  • SC environment including natural and artificial stem cells niches;
  • Modern methods of genetic engineering and gene therapy employed for SC modifications;
  • Mechanisms of SC activity following transplantation (including the role of paracrine effects);
  • Bioactive SC- derived derivatives (e.g. microvesicles; MVs) and their role in SC activity;
  • 9. Practical applications of stem cells and their derivatives in biology and medicine including heart
  • Practical classes:
  • Methods for isolation of diffrent SC fractions based on antigenic profile by MACS and FACS technolgies as well as based on adhesive properties.
  • Methods for examining multiantigenic profile of diffrent SC populations with flow cytometric platforms including non-adherent and anherent SC fractions.
  • Methods for analysis of endogenous SC- related genes expression by real time RT-PCR technology.

Requirements for passing »

The final requierments include passing the final exam (for all students) as well as accepting the final report form practical classes prepared by students working in sub-gropus.  

Final Exam (wrriten test) will include information from lectures and practical classes.

Regulations »

  • The presence during ALL practical classes is required for credits.
  • Students should prepared one final report from all three practical classes (one report per sub-group) that will be also rquired for passing.

Materials for lectures »

  • Lectures (slides given to studets during lectures as PDF files)
  • 1.http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Analysis/Flow-Cytometry/Flow-Cytometry-Technical-Resources.html?icid=fr-flow-4
  • 2. Lin KK, Goodell MA. Detection of hematopoietic stem cells by flow cytometry. Methods Cell Biol. 2011;103:21-30.
  • 3. Ratajczak MZ, Zuba-Surma EK, Machalinski B, Kucia M. Bone-marrow-derived stem cells--our key to longevity? J Appl Genet. 2007;48(4):307-19.
  • 4. Zuba-Surma EK, Ratajczak MZ. Analytical capabilities of the ImageStream cytometer. Methods Cell Biol. 2011;102:207-30.

Materials for practical classes »

(underlined to download)

Class 1. Methods for isolation of SC fractions.

INSTRUCTION to download

Additional materials:

1. Hematopoietic Stem Cell Characterization and Isolation. Lara Rossi, Grant A. Challen, Olga Sirin, Karen Kuan-Yin Lin, and Margaret A. Goodell (.pdf)

2. Analytical Capabilities of the ImageStream Cytometer. Ewa K. Zuba-Surma and Mariusz Z. Ratajczak (.pdf)

3. Bone-marrow-derived stem cells – our key to longevity?
Mariusz Z. Ratajczak, Ewa K. Zuba-Surma, Boguslaw Machalinski, Magdalena Kucia (.pdf)

Class 2. Methods for identification and phenotyping of SC populations.

INSTRUCTION to download

Class 3. Methods for molecular characterization of native and modified SC.

INSTRUCTION to download