Kurs: Introduction to Stem Cell Biology– (WBT-400E)
Coordinators:
- dr hab. Ewa Zuba-Surma, prof. UJ (lectures)
- dr hab. Ewa Zuba-Surma, prof. UJ (practical classes coordinator and leader)
- mgr Anna Łabędź-Masłowska (practical classes assistant)
Hours: 15 h lectures + 15 h practical classes
ECTS: 4
Semester: summer
Place »
- Lectures: Rm D105
- Practical classes: Rm C110
Dates »
- Lectures:
- Thursday 10.00–11.30am
- Practical classes:
- Wednesday 9.00–12.45
Initial Requirements:
This course is open for students who know English in reading, writing and speaking.
Completed "Cell Biology" course WBt-BT165 will be preffered (not applicable for international and ERASMUS students).
Passing:
- REPORT from practical classes
- Final EXAM with grade - including information from lectures and practical classes
Basic info:
- The course is dedicated for Polish as well as international students and ERASMUS Programme participants.
- All lectures, discussions and final test are given in English
Description »
Course given in ENGLISH.
The aim of the course would be to introduce basic aspects of stem cell (SC) biology and regenerative medicine to the participants .
The course will introduce: i) major populations of SC, ii) distinct sources for SC isolation including those used for clinical purposes, iii) genetic methods of SC modifications, iv) methods for SC identification and purification; v) practical applications of SC and their derivatives in regenerative medicine and for diagnostics.
- Lectures include:
- Stem/ progenitor cell types (e.g embryonic, fetal and adult SC, iPS cells);
- Sources for SC isolations including embryonic and adult tissues; (e.g. bone marrow, cord blood, mobilized peripheral blood);
- Methods for genetic reprogramming of somatic cells into iPS cells and direct progenitors;
- Approaches for identification and characterisation of rare SC populations (including the role of classical and imaging cytometry);
- SC environment including natural and artificial stem cells niches;
- Modern methods of genetic engineering and gene therapy employed for SC modifications;
- Mechanisms of SC activity following transplantation (including the role of paracrine effects);
- Bioactive SC- derived derivatives (e.g. microvesicles; MVs) and their role in SC activity;
- 9. Practical applications of stem cells and their derivatives in biology and medicine including heart
- Practical classes:
- Methods for isolation of diffrent SC fractions based on antigenic profile by MACS and FACS technolgies as well as based on adhesive properties.
- Methods for examining multiantigenic profile of diffrent SC populations with flow cytometric platforms including non-adherent and anherent SC fractions.
- Methods for analysis of endogenous SC- related genes expression by real time RT-PCR technology.
Requirements for passing »
The final requierments include passing the final exam (for all students) as well as accepting the final report form practical classes prepared by students working in sub-gropus.
Final Exam (wrriten test) will include information from lectures and practical classes.
Regulations »
- The presence during ALL practical classes is required for credits.
- Students should prepared one final report from all three practical classes (one report per sub-group) that will be also rquired for passing.
Materials for lectures »
- Lectures (slides given to studets during lectures as PDF files)
- 1.http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Analysis/Flow-Cytometry/Flow-Cytometry-Technical-Resources.html?icid=fr-flow-4
- 2. Lin KK, Goodell MA. Detection of hematopoietic stem cells by flow cytometry. Methods Cell Biol. 2011;103:21-30.
- 3. Ratajczak MZ, Zuba-Surma EK, Machalinski B, Kucia M. Bone-marrow-derived stem cells--our key to longevity? J Appl Genet. 2007;48(4):307-19.
- 4. Zuba-Surma EK, Ratajczak MZ. Analytical capabilities of the ImageStream cytometer. Methods Cell Biol. 2011;102:207-30.
Materials for practical classes »
(underlined to download)
Class 1. Methods for isolation of SC fractions.
Additional materials:
1. Hematopoietic Stem Cell Characterization and Isolation. Lara Rossi, Grant A. Challen, Olga Sirin, Karen Kuan-Yin Lin, and Margaret A. Goodell (.pdf)
2. Analytical Capabilities of the ImageStream Cytometer. Ewa K. Zuba-Surma and Mariusz Z. Ratajczak (.pdf)
3. Bone-marrow-derived stem cells – our key to longevity?
Mariusz Z. Ratajczak, Ewa K. Zuba-Surma, Boguslaw Machalinski, Magdalena Kucia (.pdf)
Class 2. Methods for identification and phenotyping of SC populations.
Class 3. Methods for molecular characterization of native and modified SC.
INSTRUCTION to download